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Test Code ARCIND ARC Indirect Titer, Blood

Methodology

Titration of Patient Plasma vs. Antigen Positive Cell

Used to monitor strength of clinically significant antibodies during pregnancy.

Testing Algorithm:

The initial test performed is an antibody identification. If the antibody problem identified is not relevant in hemolytic disease of the newborn, or if titrations are not helpful, the titer will be cancelled and will be replaced by the antibody identification test. Depending upon the antibody identification results, additional tests, ie, monospecific direct Coombs, antibody elution, antibody absorption, and red cell antigen typing may be added and charged. A consultation service is offered, at no charge, regarding the clinical relevance of red cell antibodies.

Performing Laboratory

American Red Cross

Specimen Requirements

Specimens must arrive in laboratory within 72 hours of draw.

 

Specimen Type: Blood

Container/Tube: Pink top (K2 EDTA)

Specimen Volume: Full tube

Collection Instructions: 

1. Invert several times to mix blood.

2. Avoid gross hemolysis.

3. Hand label specimens in ink with patient’s full name (first, last, and middle initial), medical record number or date of birth, date and time of draw, and initials of collector.

4. Serum gel tube is not acceptable.

Additional Information: Indicate pregnancy/delivery due date.

Specimen Transport Temperature

Ambient <72 hours/ Refrigerate (1-8° C) >72 hours

Reference Values

Negative
If positive, result will be reported as the reciprocal of the highest dilution at which macroscopic agglutination (1+) is observed. Consultative report is included.

Day(s) Test Set Up

Monday through Friday

Test Classification and CPT Coding

86870-Antibody identification
86860-Antibody elution (if appropriate)
86880 x 3-Antiglobulin, direct (if appropriate)
86886-Antibody titer (if appropriate)
86905-Each red cell antigen typing (if appropriate)
86978-Absorption, each (if appropriate)