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HelpTest Code BCRFX BCR::ABL1 Qualitative Diagnostic Assay with Reflex to BCR::ABL1 p190 Quantitative Assay or BCR::ABL1 p210 Quantitative Assay, Varies
Ordering Guidance
This test should not be used to monitor BCR::ABL1 fusion forms.
To monitor patients carrying BCR::ABL1 fusion forms coding for the p190 (e1/a2) protein, order BA190 / BCR::ABL1, p190, mRNA Detection, Reverse Transcription-PCR (RT-PCR), Quantitative, Monitoring Assay, Varies.
To monitor patients carrying BCR::ABL1 fusion forms coding for the p210 (e13/a2 or e14/a2) protein, order BCRAB / BCR::ABL1, p210, mRNA Detection, Reverse Transcription-PCR (RT-PCR), Quantitative, Monitoring Chronic Myeloid Leukemia (CML), Varies.
To monitor patients carrying rare BCR::ABL1 fusion forms coding for e19a2, e13/e14a3, e1a3, e6a2, e19a3, e8a2, e12a2, e6a3, e8a3, and e12a3, order BARQ / BCR::ABL1, Rare Fusion Monitoring, Quantitative, Varies.
If the patient has a previous positive history of p190, p210, or rare fusion forms, this test will be cancelled and one of the following appropriate monitoring tests will be added:
-BA190 / BCR::ABL1, p190, mRNA Detection, Reverse Transcription-PCR (RT-PCR), Quantitative, Monitoring Assay, Varies
-BCRAB / BCR::ABL1, p210, mRNA Detection, Reverse Transcription-PCR (RT-PCR), Quantitative, Monitoring Chronic Myeloid Leukemia (CML), Varies
-BARQ / BCR::ABL1, Rare Fusion Monitoring, Quantitative, Varies
If the specimen is greater than 72 hours post-collection when received, this test will be canceled and BADX / BCR::ABL1, Qualitative, Diagnostic Assay, Varies will be added and performed as the appropriate test if the specimen is still within stability for that test.
Shipping Instructions
Specimens must arrive within 72 hours of collection. Collect and package specimen as close to shipping time as possible.
Necessary Information
Pertinent clinical history including if the patient has a diagnosis of chronic myeloid/myelogenous leukemia or other BCR::ABL1 positive neoplasm is required.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Whole blood
Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Yellow top (ACD)
Specimen Volume: 10 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimen in original tube. Do not aliquot.
3. Label specimen as whole blood.
Specimen Type: Bone marrow
Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Yellow top (ACD)
Specimen Volume: 4 mL
Collection Instructions:
1. Invert several times to mix bone marrow.
2. Send bone marrow specimen in original tube. Do not aliquot.
3. Label specimen as bone marrow.
Forms
1. Hematopathology Patient Information (T676)
2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.
Useful For
Diagnostic workup of patients with high probability of BCR::ABL1-positive hematopoietic neoplasms, predominantly chronic myeloid/myelogenous leukemia and acute lymphoblastic leukemia
Testing Algorithm
If the initial qualitative testing is negative, no additional testing will be performed.
If the initial qualitative testing is positive for the common p190 or p210 BCR::ABL1 forms, reflex testing to determine the quantitative transcript level of the relevant messenger RNA will be performed at an additional charge. A normalized percentage of transcript level is provided in an integrated report. For the p210 target, the value is additionally defined using the international scale convention. These integrated results provide a baseline quantitative transcript to monitor treatment response.
If the initial qualitative testing is positive for an alternate rare form of BCR::ABL1, then BARQ / BCR::ABL1 Rare Fusion Monitoring, Quantitative, Varies will be added and performed at an additional charge, with results reported separately.
For more information see:
-Myeloproliferative Neoplasm: A Diagnostic Approach to Bone Marrow Evaluation
-Myeloproliferative Neoplasm: A Diagnostic Approach to Peripheral Blood Evaluation
Special Instructions
Method Name
Reverse Transcription Polymerase Chain Reaction (RT-PCR) Multiplex PCR
Reporting Name
BCR/ABL1 Reflex, Qual/QuantSpecimen Type
VariesSpecimen Minimum Volume
Whole Blood: 8 mL; Bone marrow: 2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Refrigerated (preferred) | 72 hours | PURPLE OR PINK TOP/EDTA |
| Ambient | 72 hours | PURPLE OR PINK TOP/EDTA |
Reject Due To
| Gross hemolysis | Reject |
| Moderately to severely clotted | Reject |
Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Saturday
Report Available
7 to 10 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
81206
81207
81208
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BCRFX | BCR/ABL1 Reflex, Qual/Quant | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| MP039 | Specimen Type | 31208-2 |
| 48389 | BCR/ABL1 Reflex Result | No LOINC Needed |
| 48388 | Interpretation | 69047-9 |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| B190R | BCR/ABL1, p190, Quant, Reflex | Yes, (order BA190) (Bill Only) | No |
| B210R | BCR/ABL1, p210, Quant, Reflex | Yes, (order BCRAB) (Bill Only) | No |