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HelpTest Code BRBPS Broad Range Bacterial PCR and Sequencing, Varies
Useful For
Detecting and identifying bacteria (including mycobacteria) from normally sterile sources, including synovial fluid; body fluids such as pleural, peritoneal, pericardial, and cerebrospinal fluid; and both fresh and formalin-fixed paraffin-embedded tissues
This test is not recommended as a test of cure because nucleic acids may persist for long periods of time after successful treatment.
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ISNGS | Ident by Next Generation Sequencing | No, (Bill Only) | No |
| SPID2 | Specimen Identification by PCR | No, (Bill Only) | No |
| CSFME | Meningitis Encephalitis Panel, PCR | Yes | No |
| JIP | Joint Infect Panel PCR, Synovial Fl | Yes | No |
Testing Algorithm
If polymerase chain reaction (PCR) test results are negative, no next-generation sequencing is performed.
If PCR testing is positive, next-generation sequencing is performed.
The following algorithms are available:
-Infective Endocarditis: Diagnostic Testing for Identification of Microbiological Etiology
Method Name
Polymerase Chain Reaction (PCR) followed by Next-Generation Sequencing
Reporting Name
Broad Range Bacteria PCR+SequencingSpecimen Type
VariesNecessary Information
Specimen source is required.
Specimen Required
Fresh tissue is generally preferred over formalin-fixed, paraffin-embedded tissue, but either can be tested.
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Fresh tissue or biopsy
Sources: Normally sterile tissue such as bone, lymph node, joint, heart valve, brain, viscera, organ, lung, prostate
Container/Tube: Sterile container
Specimen Volume: Entire collection or 5 mm(3)-approximately the size of a pencil eraser
Collection Instructions:
1. Collect fresh tissue specimen.
2. Submit tissue only, do not add fluid (eg, saline, broth, formalin, formaldehyde, acetone) to tissue. Body fluid accompanying tissue is acceptable.
3. Freeze specimen.
Specimen Stability Information: Frozen (preferred) 21 days/Refrigerated 21 days
Acceptable:
Preferred: Paraffin-embedded tissue block
Supplies: Tissue Block Container (T553)
Specimen Type: Formalin-fixed, paraffin-embedded (FFPE) tissue block
Source: Normally sterile or deep tissues such as bone, lymph node, joint, heart valve, brain, viscera, organ, lung, prostate
Container/Tube: Tissue block
Collection Instructions: Submit a formalin-fixed, paraffin-embedded tissue block to be cut and returned.
Specimen Stability Information: Ambient (preferred)/Refrigerated
Acceptable: Paraffin-embedded tissue block
Specimen Type: Section (scrolls) of FFPE tissue block
Source: Normally sterile or deep tissues such as bone, lymph node, joint, heart valve, brain, viscera, organ, lung, prostate
Container/Tube: Sterile container for each individual cut section (scroll)
Collection Instructions: Perform microtomy and prepare five separate 10-micron sections. Each section (scroll) must be placed in a separate sterile container for submission.
Specimen Stability Information: Ambient (preferred)/Refrigerated
Specimen Type: Fluid
Sources: Normally sterile body fluids such as vitreous humor, pleural, abdominal, peritoneal, ascites, pericardial, pelvic, prostatic
Container/Tube: Screw-capped, sterile container
Specimen Volume: 0.5 mL
Collection Instructions:
1. Collect fresh fluid specimen.
2. Freeze specimen.
Specimen Stability Information: Frozen (preferred) 21 days/Refrigerated 21 days
Specimen Type: Spinal fluid
Container/Tube: Screw-capped, sterile container
Specimen Volume: 0.5 mL
Collection Instructions:
1. Collect fresh spinal fluid (CSF) specimen using sterile technique.
2. Submit specimen from collection vial 2 or higher, specimens in vial 1 are not acceptable.
3. Indicate on the label which vial is being submitted.
4. CSF collected by lumbar puncture is preferred. CSF collected via shunt and ventricular fluid are also acceptable; label tube with applicable collection information if submitting nonlumbar puncture collected CSF.
Specimen Stability Information: Frozen (preferred) 21 days/Refrigerated 21 days
Specimen Type: Synovial fluid
Container/Tube:
Preferred: Red top or sterile container
Acceptable: Lavender top (EDTA), pink top (EDTA), royal blue top (EDTA), or sterile vial containing EDTA-derived aliquot
Specimen Volume: 0.5 mL
Collection Instructions: Send specimen in original tube (preferred).
Specimen Stability Information: Frozen (preferred) 21 days/Refrigerated 21 days
Specimen Minimum Volume
Fluid: 0.2 mL; Fresh tissue or biopsy: 5 mm(3); Paraffin-embedded tissue block: two 10-micron sections
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Varies | |
Reject Due To
| Tissue received in any exogenous fluid (eg, saline, broth, formalin, formaldehyde, acetone) | Reject |
| Specimen received in anaerobe vial | Reject |
| Wrapping (gauze, drapes, etc) | Reject |
| Blood Culture bottles (Bactec FX or BacT/ALERT bottles) | Reject |
| Bone marrow | Reject |
| Decalcified bone | Reject |
| Slides | Reject |
| Skin biopsy | Reject |
| Colon biopsy | Reject |
| Formalin-fixed paraffin-embedded body fluid | Reject |
Reference Values
No bacterial DNA detected
Day(s) Performed
Monday through Friday
Report Available
14 to 28 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87801-Broad Range Bacterial PCR and Sequencing
87798-Specimen Identification by PCR (if appropriate)
87798-Ident by Next Generation Sequencing (if appropriate)
87483-Meningitis Encephalitis Panel, PCR (if appropriate)
87999-Joint Infection Panel, PCR, Synovial Fluid (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BRBPS | Broad Range Bacteria PCR+Sequencing | 76575-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| BRBPS | Broad Range Bacteria PCR+Sequencing | 76575-0 |
Special Instructions
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.