Test Code CBFAERANA Culture, Aerobic and Anaerobic, Body Fluid
Methodology
Presumptive and definitive identification of microorganisms by Gram stain and colony morphology growth on selective media and biochemical panels.Includes susceptibilities as needed at an additional charge.
All body fluid requests will automatically have a Gram stain added. This will allow us to better assess the need for special culture procedures and thus ensure a more accurate result, while at the same time providing pertinent information to the physician as early as possible.
Performing Laboratory
Sky Lakes Medical Center-MicrobiologySpecimen Requirements
Acceptable Specimens: Peritoneal fluid, pleural fluidUnacceptable Specimens: Drainage sites or pus (order wound culture), urine (order urine culture)
Specimen source is required.
Specimen must arrive in laboratory within 24 hours of collection.
Preferred
Specimen Type: Body fluid
Container/Tube: Sterile container or syringe
Specimen Volume: 1 mL
Specimen Minimum Volume: 0.5 mL
Collection Instructions:
1. If transport in syringe, the needle must be removed prior to transport to laboratory.
2. Label container/syringe with patient’s name (first and last), medical record number or date of birth, specimen source, and date and time of collection.
3. As with other cultures, administration of antibiotics prior to culture may make recovery of pathogens difficult to impossible.
4. Rejection criteria include delayed transport of >24 hours at ambient temperature, unsterile container, syringe with needle attached, or specimen in EDTA tube.
Alternate
Specimen Type: Swab
Container/Tube: Culture transport swab in sterile culture transport tube
Specimen Volume: Swab
Collection Instructions:
1. Label tube with patient’s name (first and last), medical record number or date of birth, specimen source, and date and time of collection.
2. As with other cultures, administration of antibiotics prior to culture may make recovery of pathogens difficult to impossible.
3. Rejection criteria include delayed transport of >24 hours at ambient temperature, unsterile container, syringe with needle attached, or specimen in EDTA tube.
Additional Information: Swab specimen is not recommended unless it’s the only available method.
Specimen Transport Temperature
AmbientReference Values
NegativeDay(s) Test Set Up
Monday through SundayTest Classification and CPT Coding
87070-Culture87205-Gram stain