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Test Code DIRCOO/DAT Coombs, Direct, Blood

Methodology

Antihuman Globulin (AHG)/Polyspecific

When positive, monospecific anti-IgG and monospecific anti-C3b, C3d (Complement) will be performed, except on newborns which are tested only with anti-IgG.

Performing Laboratory

Sky Lakes Medical Center-Blood Bank

Specimen Requirements

Specimen Type: Blood

Container/Tube: Pink top (K2 EDTA)

Specimen Volume: Full tube

Collection Instructions: 

1. Invert several times to mix blood.

2. Hand label specimen in ink with patient’s full name (first, last, and middle initial), medical record number or date of birth, date and time of draw, and initials of collector.

3. Avoid gross hemolysis.

4. Serum gel tube is not acceptable.

Specimen Transport Temperature

Ambient

Reference Values

Negative

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding

86880-Antihuman globulin (Coombs’) test
86880 x 2-Additional Coombs’ test (if appropriate)